Recent Publications                                                                               

Nag S, Manias JL, Stewart DJ. Pathology and new players in the pathogenesis of brain edema.

Acta  Neuropathol. 2009. DOI 10.1007/s00401-009-0541-0

Nag S, Manias JL, Stewart DJ. Blood-brain barrier breakdown is associated with increased expression

of phosphorylated caveolin-1 in endothelium.  Neuropathol Appl Neurobiol. 35:417-426, 2009

Yeung D, Manias J, Stewart DJ, Nag S. Decreased junctional adhesion molecule – A expression during

blood-brain barrier breakdown. Acta Neuropathol 115:635-642, 2008

Nag S, Venugopalan R, Stewart DJ. Increased caveolin-1 expression precedes alterations in tight

junction proteins during blood-brain barrier breakdown. Acta Neuropathol 114(5):459-469, 2007

Hoffman WH, Casanova MF, Cudrici CD, Zakranskaia E, Venugopalan R, Nag S, Oglesbee MJ, Rus H.

Neuroinflammatory response of the choroid plexus epithelium in fatal diabetic ketoacidosis.

Exp Mol Pathol 83:65-72, 2007

Nag S, Nourhaghighi N, Davis J, Asa S, Stewart DJ. Angiopoietins are expressed in the normal pituitary gland.

Endocrine Pathology 16 1:67-74, 2005

Nag S, Papneja T, Venugopalan R, Stewart DJ. Increased expression of angiopoietin2 is associated with

endothelial apoptosis and blood-brain barrier breakdown. Lab Invest 85,10:1189-1198, 2005

Books

                   

COMING SOON !  Currently in preparation...

The Blood-Brain and Other Neural Barriers:  Biology and Research Protocols

Editor: Sukriti Nag

Contents

BIOLOGY OF THE BARRIERS

1.        Morphology and Properties of the Neurovascular Unit: Cerebral Endothelium.  Sukriti Nag

2.        Morphology and Properties of the Neurovascular Unit: Pericytes.  Paula Dore-Duffy and Kristen Cleary

3.        Morphology and Properties of the Neurovascular Unit: Astrocytes.  Sukriti Nag

4.        The Blood-CSF Barrier: Structure and Functional Significance.  Conrad Johanson

5.        The Blood-Retinal Barrier: Structure and Functional Significance.  E. Aaron Runkle and David A. Antonetti

6.        The Blood-Nerve Barrier: Structure and Functional Significance. Ananda Weerasuriya

RESEARCH PROTOCOLS

PART I:  IMAGING THE BARRIERS

7.          Detection of Multiple Proteins in Intracerebral Vessels by Confocal Microscopy. Qualitative and Quantitative Studies

        Janet L. Manias, Anish Kapadia, Sukriti Nag

8.        Multiparametric Magnetic Resonance Imaging and Repeated Measurements of BBB Permeability to Contrast Agents 

        Tavarekere N Nagaraja, Robert A. Knight, James R. Ewing, Kishore Karki, Vijaya Nagesh and Joseph D. Fenstermacher

9.          Detection of Brain Pathology by Magnetic Resonance Imaging of Iron Oxide Microparticles

        Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis and Robin P. Choudhury

10.      Measuring the Integrity of the Human BBB Using Magnetic Resonance Imaging - Andrea Kassner, Rebecca Thornhill

11.      Measuring Blood-Cerebrospinal Fluid Barrier Permeability in the Rat Embryo.

        Norman R. Saunders, C.J. Ek, MD Habgood, PA Johansson, S Liddelow, KM Dziegielewska

12.      Detection of Blood-Nerve Barrier Permeability by Magnetic Resonance Imaging.  Carsten Wessig

 PART II: MOLECULAR TECHNIQUES TO STUDY THE BLOOD-BRAIN BARRIER

13.   Isolation and Characterization of Lipid Raft Proteins in Human Brain Endothelial Cells by Mass Spectroscopy

        Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers and Alexandre Prat

14.   Analysis of Mouse Brain Microvascular Endothelium Using Laser Capture Microdissection Coupled with Proteomics

        Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan L, Shiae-Lin Wu, William S. Hancock and Joel S. Pachter

15.   In vitro Studies of BBB Transporter proteins: Molecular expression and Functional Activity.  G. Chan and Reina Bendayan

16.  Methods to study glycoproteins at the blood-brain barrier using mass spectrometry.

        Arsalan S. Haqqani, Jennifer J. Hill, James Mullen and Danica B. Stanimirovic

 PART III: MODELS TO STUDY THE BARRIERS     

17.   Characterization of Septate Junctions of the Drosophila: Novel Models for Studies of the Blood-Brain and Blood-Eye Barriers

        Robert L. Pinsonneault, Nasima Mayer, Fahima Mayer,  Nebiyu Tegegn,  and Roland J. Bainton

18.   The zebrafish model of the Blood-Brain Barrier: Morphological and Permeability Studies

        Brian P. Eliceiri, Ana Maria Gonzalez and Andrew Baird

19.   Methods to Assess Pericyte-Endothelial Cell Interaction in Matrix Metalloproteinase Secretion.

        Gokulan Thanabalasundaram, Jehad El-Gindi, Mira Lischper and Hans-Joachim Galla

20.   Isolation and Properties of an In Vitro Human Outer Blood-Retinal Barrier Model. Lopa Leach

21.   Isolation and Properties of Endothelial Cells Forming the Blood-Nerve Barrier  Yasuteru Sano and Takashi Kanda 

 PART IV: DELIVERY OF THERAPEUTIC AGENTS ACROSS THE BARRIERS

22.   Treatment of Focal Brain Ischemia with Viral Vector-Mediated Gene Transfer.   Hua Su and Guo-Yuan Yang

23.   BBB Disruption in the Treatment of Brain Tumors.  Marie Blanchette and David Fortin

24.   Integrated Platform for Brain Imaging and Drug Delivery Across the Blood-Brain Barrier

        Umar Iqbal, Abedelnasser Abulrob and Danica Stanimirovic

25.   Targeting the Choroid Plexus-CSF-brain Nexus Using Peptides Identified by Phage Display

        Andrew Baird, Brian Eliceiri, Ana Maria Gonzalez, Conrad Johanson, Wendy Leadbeater and Edward Stopa