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Professor Department of Physiology |
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Anthony Gramolini, PhD |
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Department of Physiology Ted Rogers Centre for Heart Research Translational Biology and Engineering Program 661 University Ave, Rm 1437 TMDT#2, Mars2 Tower Toronto, ON Canada, M5G1M1 Phone: 416-946-8257 |
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A major interest of our lab is to investigate the cellular mechanisms involved in the regulation of calcium cycling at the level of sarcoplasmic reticulum and its role in cardiac disease. For these experiments, we apply conventional biochemical and molecular biology assays together with digital confocal imaging. We are also applying advanced proteomic detection methods as well as protein-protein interaction methods to decipher critical events and functions in cardiac and skeletal muscle. Model systems include established cell lines, primary cell cultures, human cardiac stem cells and transgenic animals, along with human explants and tissues. These techniques serve as valuable experimental tools to investigate cell biology and cellular pathways. Below is a general overview of the major proteins involved in muscle excitation-contraction. Our research interests focus on proteins involved in Ca2+ release from the sarcoplasmic reticulum and its subsequent reuptake.
Considerable effort has gone into developing new approaches in studying cardiac health and function. Specifically we have been very interested in identifying new proteins and pathways in cardiac myocytes.
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University of Toronto, Toronto, Ontario, M5G 1M1 |