DDx – high background

· Slides not sufficiently cleaned prior to sample prep

· Try dipping in EtoH and wipe dry using lint-free paper prior to dropping slides

· Cellular debris in the cell suspension / sample preparation

· Try washing x 3

· Slide was aged by baking

· Try increasing denaturation time to 10 minutes

· slide contains cytoplasm

· try increasing denaturation time to 10 minutes

· wash step inadequate

· ensure pH and temperature of wash solutions are correct

· repeat the wash procedure

· change wash solutions appropriately

References:

· Vysis package insert