QF-PCR

• Application to prenatal aneuploidy diagnosis:
• STR’s (at least 2 informative per chromosome)
1. Informativeness generally > 98% (Hultén et al, 2003)
• sensitivity:
1. 99.7% overall (Hultén et al, 2003)
• specificity:
1. false positive is rare (Hulten et al, 2003)
1. main problem is sex chromosomes
• mosaicism is not reliably detected

References:

• Hultén MA, Dhanjal S, Pertl B. Rapid and simple prenatal diagnosis of common chromosome disorders: advantages and disadvantages of the molecular methods FISH and QF-PCR. Reproduction (2003);126:279-297.