DDx - overspreading

· Cell suspension is not concentrated enough

· Slide-making technique:

· Height of slide dropping too high

· Slide-making conditions:

· Temperature too low

· Humidity is too high

· Not enough air flow

References:

· Barch MJ, Knutsen T, Spurbeck JL. The AGT Cytogenetics Laboratory Manual. 3rd ed. Lippincott Williams & Wilkins; 1997.