MOFFAT LAB
UNIVERSITY OF TORONTO
Publications
- Moffat, J., Reiling, J., and Sabatini, D.M. (2007) OTEs associated with long dsRNAs in Drosophila RNAi screens. Trends in Pharmacological Sciences, 28(4): 149-151.
- Guertin, D.A., Stevens, D.M., Thoreen, C.C., Burds, A.A., Kalaany, N.Y., Moffat, J., Brown, M., Fitzgerald, K.J., and Sabatini, D.M. (2006) Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCa, but not S6K1. Developmental Cell, 11(6): 859-871.
- Carpenter, A.E., Jones, T.R., Lamprecht, M., Wheeler, D.B., Clarke, C., Kang, I.H., Friman, O., Guertin, D., Ma, S., Chang, J.H., Lindquist, R., Moffat, J., Gollard, P., and Sabatini, D.M. (2006) CellProfiler: Image Analysis Software for Identifying and Quantifying Cell Phenotypes. Genome Biology, 7(10): R100.
- Moffat, J., Grueneberg, D.A., Yang, X., Kim, S.Y., Kloepfer, A.M., Hinkle, G., Piqani, B., Eisenhaure, T.M., Luo, B., Grenier, J.K., Carpenter, A.E., Foo, S.Y., Stewart, S.A., Stockwell, B.R., Hacohen, N., Hahn, W.C., Lander, E.S., Sabatini, D.M., and Root, D.E. (2006) A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 124(6): 1283-1298.
- Moffat, J. and Sabatini, D.M. (2006) Building mammalian signalling pathways with RNAi screens. Nature Reviews Molecular & Cellular Biology 7(6): 177-187. (Featured article)
- *Mnaimneh, S., *Davierwala, A.P., *Haynes, J., Moffat, J., Peng, W.-T., Zhang, W., Yang, X., Pootoolal, J., Chua, G., Lopez, A., Trochesset, M., Morse, D., Krogan, N., Hiley, S.L., Li, Z., Morris, Q., Grigull, J., Mitsakakis, N., Roberts, C.J., Greenblatt, J.F., Boone, C., Kaiser, C.A., Andrews, B.J., and Hughes, T.R. (2004). Exploration of essential gene functions via titratable promoter alleles. Cell 118(1): 31-44.
- Moffat, J. and Andrews, B. (2004) Late-G1 cyclin–CDK activity is essential for control of cell morphogenesis in budding yeast. Nature Cell Biology 6(1): 59-66.
- Willis, K., Barbara, K.E., Menon, B.B., Moffat, J., Andrews, B., Santangelo, G. (2003) Glucose-dependent CLN function in G1 and G2/M requires stimulation of translation by the transcriptional activator Gcr1p. Genetics 165: 1017-1029.
- Moffat, J. and Andrews, B. (2003) Ac’septin’ a signal: kinase regulation by septins. Developmental Cell 5: 528-530.
- *Huang D., *Moffat J., Andrews B. (2002) Dissection of a complex phenotype by functional genomics reveals roles for the yeast cyclin-dependent protein kinase Pho85 in stress adaptation and cell integrity. Mol. Cell. Biol. 22(14): 5076-88. (*equal contributions, see acknowledgements).
- McBride H.J., Sil A., Measday V., Yu Y., Moffat J., Maxon M.E., Herskowitz I., Andrews B., Stillman D.J. (2001) The protein kinase Pho85 is required for asymmetric accumulation of the Ash1 protein in Saccharomyces cerevisiae. Mol. Microbiol. 42(2):345-53.
- Baetz K., Moffat J., Haynes J., Chang M., Andrews B. (2001) Transcriptional coregulation by the cell integrity mitogen-activated protein kinase Slt2 and the cell cycle regulator Swi4. Mol. Cell. Biol. 21(19):6515-28.
- Moffat J., Huang D., Andrews B. (2000) Functions of Pho85 cyclin-dependent kinases in budding yeast. Prog. Cell Cycle Res. 4:97-106.
- Measday V., McBride H., Moffat J., Stillman D., Andrews B. (2000) Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast. Mol. Microbiol. 35(4):825-34.
- Huang D., Patrick G., Moffat J., Tsai L.H., Andrews B. (1999) Mammalian Cdk5 is a functional homologue of the budding yeast Pho85 cyclin-dependent protein kinase. Proc. Natl. Acad. Sci. U.S.A. 96(25):14445-50.
- Huang D., Moffat J., Wilson W.A., Moore L., Cheng C., Roach P.J., Andrews B. (1998) Cyclin partners determine Pho85 protein kinase substrate specificity in vitro and in vivo: control of glycogen biosynthesis by Pcl8 and Pcl10. Mol. Cell. Biol. 18(6):3289-99.
- Huyer G., Kelly J., Moffat J., Zamboni R., Jia Z., Gresser M.J., Ramachandran C. (1998) Affinity selection from peptide libraries to determine substrate specificity of protein tyrosine phosphatases. Anal. Biochem. 258(1):19-30.
- Kennedy B.P., Soravia C., Moffat J., Xia L., Hiruki T., Collins S., Gallinger S., Bapat B. (1998) Overexpression of the nonpancreatic secretory group II PLA2 messenger RNA and protein in colorectal adenomas from familial adenomatous polyposis patients. Cancer Res. 58(3):500-3.
- Huyer G., Liu S., Kelly J., Moffat J., Payette P., Kennedy B., Tsaprailis G., Gresser M.J., Ramachandran C. (1997) Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. J. Biol. Chem. 272(2):843-51.