Movies
Germband cells in embryos expressing E-cadherin:GFP (green) and myosin:mCherry (magenta) under sham irradiation (left) or upon wounding and apical constriction of the cells anterior and posterior to a multicellular vertex (right). Arrows indicate resolving multicellular vertices. A stack was acquired every 3 s. Time is indicated as min:s. Anterior left, dorsal up.
Source: Yu JC, Fernandez-Gonzalez R. Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila. eLife 2016 Jan 9;5.
Epidermal cells expressing ubi–E-cadherin:GFP (green) and myosin:mCherry (red) in a control embryo injected with 50% DMSO (left) or 50 mM dynasore (right). Images were acquired by time-lapse confocal microscopy using a spinning disk confocal microscope (Revolution XD; Andor Technology). A stack was acquired every 30 s for 44 min. Time after wounding is shown. Anterior left, dorsal up.
Source: Hunter MV, Lee DM, Harris TJC, Fernandez-Gonzalez R. Polarized E-cadherin endocytosis directs actomyosin assembly during embryonic wound repair. J Cell Biol 2015 Aug 31;210(5):801-16.
4D movie of cellular dynamics during wound closure from apical (left) and lateral (right) perspectives.
Source: Zulueta-Coarasa T, Tamada M, Lee EJ, Fernandez-Gonzalez R. Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair. Development. 2014 Jul;141(14):2901-11.
Epidermal cells expressing myosin:mCherry (left) and Abl:GFP (right). Time after wounding is indicated. Anterior left, dorsal up.
Source: Zulueta-Coarasa T, Tamada M, Lee EJ, Fernandez-Gonzalez R. Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair. Development. 2014 Jul;141(14):2901-11.
Myosin accumulates in medial networks in wounded epidermal cells of the early Drosophila embryo. Epidermal cells expressing E-cadherin:GFP (green) and myosin:mCherry (red) in a stage 7 embryo. The time after wounding is indicated. Anterior left, dorsal up.
Source: Fernandez-Gonzalez R, Zallen JA. Wounded cells drive rapid epidermal repair in the early Drosophila embryo. Mol Biol Cell. 2013 Oct;24(20):3227-37.
Myosin forms a purse string around wounded epidermal cells in the late Drosophila embryo. Epidermal cells expressing E-cadherin:GFP (green) and myosin:mCherry (red) in a stage 14 embryo. The time after wounding is indicated. Anterior left, dorsal up.
Source: Fernandez-Gonzalez R, Zallen JA. Wounded cells drive rapid epidermal repair in the early Drosophila embryo. Mol Biol Cell. 2013 Oct;24(20):3227-37.
Rodrigo was interviewed by the U of T Faculty of Engineering!