Sixth International Workshop on Arylamine N-Acetyltransferases

Keynote Speaker

We are pleased to announce that our opening Keynote Lecture will be presented by Jack Uetrecht, MD, PhD.

Dr. Uetrecht received his Ph.D. in organic chemistry from Cornell University, his M.D. at Ohio State University and did his internal medical residency at the University of Kansas Medical Center. He completed his clinical pharmacology fellowship in 1981 at Vanderbilt University and moved to the University of Toronto in 1985, where he is currently Professor of Pharmacy and Medicine and the Canada Research Chair in Adverse Drug Reactions at the University of Toronto. Dr. Uetrecht is on the editorial boards of Chemical Research in Toxicology, Current Drug Metabolism, Drug Metabolism Reviews, Expert Opinion on Drug Metabolism and Toxicology, and Drug Metabolism Letters. He chaired the Health Canada Scientific Advisory Panel for Hepatotoxicity, and has appeared before the FDA and the EMA on several occasions. He received the Janssen-Ortho Research Award in 2001, the McEwan Lectureship in 2007, and is a Fellow of the Canadian Academy of Health Sciences. His research program is focused on understanding the mechanisms of idiosyncratic drug reactions.

Dr. Uetrecht's Keynote Lecture is entitled "Why are Aromatic Amines and Hydrazines Structural Alerts for Drug Development?"

Detailed Program

Friday, October 4th - Afternoon

12:00	Registration Open
13:00 - 13:15	Opening Welcome Denis Grant, University of Toronto
13:15 - 14:00	Keynote Lecture Jack Uetrecht, University of Toronto Why are Aromatic Amines and Hydrazines Structural Alerts for Drug Development?
14:00 - 14:45	Perspectives Lecture Charlene McQueen, US Environmental Protection Agency The Arylamine N-Acetyltransferases (NAT) Conundrum: Solving the Puzzle
14:45 - 15:15	Refreshment Break
15:15 - 17:15	Session I: NAT Pharmacogenetics, Polymorphisms and Disease Risk Chair: David Hein, University of Louisville
15:15 - 15:45	David Hein, University of Louisville, Louisville KY, USA NAT2 Polymorphisms and Disease Risk: Implications of Genetic Heterogeneity in the Slow Acetylator Phenotype
15:45 - 16:15	Klaus Golka, Leibnitz Research Center, Dortmund, Germany NAT110 in Bladder Cancer Patients*
16:15 - 16:45	Raul Salazar-Gonzalez, UASLP, Mexico Increased NAT2 Expression in NK cells and its Gene Polymorphisms Related to Anti-tuberculosis Therapy in Mexican mestizo Population
16:45 - 17:15	Mark Doll, University of Louisville, Louisville, KY, USA Acetylator Genotype-Dependent N-Acetylation of Isoniazid in Human Hepatocytes In Situ
17:15 - 18:00	Session II: Update on NAT Gene Nomenclature Chair: Sotiria Boukouvala, Democritus University of Thrace, Alexandroupolis, Greece
18:00	Opening Reception

Saturday, October 5th - Morning

08:30 - 09:00	Buffet Breakfast
09:00 - 10:30	Session III: Metabolism and Toxicity of Aromatic Amines I Chair: Kim Sugamori, University of Toronto, Toronto, Canada
09:00 - 09:30	Daniel Hanna, University of Toronto, Toronto, Canada Influence of Sex and Strain on Hepatotoxic and Immune Responses in a Model of Chemical-Induced Liver Carcinogenesis in the Mouse
09:30 - 10:00	Marcus Stepp, University of Louisville, Louisville KY, USA Rapid Arylamine N-Acetyltransferase Congenic Rats Show Increase of Chemically Induced Breast Tumors by N-Methylnitrosourea
10:00 - 10:30	Imir Metushi, University of Toronto, Toronto, Canada A Fresh Look at Isoniazid-Induced Liver Injury: Isoniazid is Directly Oxidized to a Reactive Metabolite that Induces an Immune Response
10:30 - 11:00	Refreshment Break
11:00 - 12:30	Session IV: Novel Functions of NAT Proteins Chair: Edith Sim, University of Kingston, Kingston, UK
11:00 - 11:30	James Egleton, University of Oxford, Oxford, UK Colorimetric Probes for Specific Detection of Breast Cancer Biomarker hNAT1
11:30 - 12:00	Katey Witham, University of Queensland, Brisbane, Australia The Effect of Human NAT1 on the Methionine Salvage Pathway in Cancer Cells
12:00 - 12:30	Edith Sim, University of Kingston, Kingston, UK From Arylamine N-Acetyltransferase to Folate-Dependent Acetyl CoA Hydrolase: Impact of Folic Acid on the Activity of Human NAT1 and its Murine Homologue Nat2
12:30 - 13:30	Lunch and Posters

Saturday, October 5th - Afternoon

13:30 - 15:30	Session V: NAT Structure-Function Relationships Chair: Fernando Rodrigues-Lima, University of Paris Diderot, Paris, France
13:30 - 14:00	Neville Butcher, University of Queensland, Brisbane, Australia Auto-Acetylation of Human NAT1 and the Effects of Sirt1 and Sirt2
14:00 - 14:30	Rodney Minchin, University of Queensland, Brisbane, Australia Role of Lysine100 in the Catalytic Activity of Human NAT1
14:30 - 15:00	Edith Sim, University of Kingston, Kingston, UK Production of Recombinant Mouse Arylamine N-Acetyltransferase Type 1 and Specificity Comparison with Other Mammalian NAT Enzymes
15:00 - 15:30	Fernando Rodrigues-Lima, University of Paris Diderot, Paris, France Structure of an Active Arylamine N-Acetyltransferase Possessing a Non-Canonical Catalytic Triad
15:30 - 16:00	Refreshment Break
16:00 - 17:30	Session VI: Functions and Regulation of Mammalian NAT Enzymes Chair: Brunhilde Blomeke, University of Trier, Trier, Germany
16:00 - 16:30	Sotiria Boukouvala, Democritus University of Thrace, Alexandroupolis, Greece A Comparative Functional Investigation of NATs in Primates
16:30 - 17:00	Andrew Paterson, Hospital for Sick Children, Toronto, Canada Acetylation Status of N-Acetyltransferase 2 (NAT2) is Associated with Skin Fluorescence in Subjects With and Without Type 1 Diabetes
17:00 - 17:30	Brunhilde Blomeke, University of Trier, Trier, Germany NAT Expression in Skin: Impact on Aromatic Amines
18:00 - 21:00	Workshop Dinner

Sunday, October 6th - Morning

08:00 - 08:30	Breakfast
08:30 - 10:00	Session VII: NAT in Microorganisms Chair: Jean-Marie Dupret, University of Paris Diderot, Paris, France
08:30 - 09:00	Sotiria Boukouvala, Democritus University of Thrace, Alexandroupolis, Greece Microbial NATs of Agricultural and Ecological Significance
09:00 - 09:30	Areej Abuhammad, University of Oxford, Oxford, UK NAT as a Target for Anti-Tubercular Drug Therapy: New Mechanisms of Inhibition
09:30 - 10:00	Jean-Marie Dupret, University of Paris Diderot, Paris, France Biotransformation of Aromatic Amines by Trichoderma spp.
10:00 - 10:30	Refreshment Break
10:30 - 12:30	Session VIII: Metabolism and Toxicity of Aromatic Amines II Chair: Charlene McQueen, US Environmental Protection Agency
10:30 - 11:00	Shuang Wang, University of Toronto, Toronto, Canada N-Hydroxylation of 4-Aminobiphenyl by Cytochrome P450 2E1 Produces Oxidative Stress in a Mouse Model of Chemically-induced Liver Cancer
11:00 - 11:30	Florent Busi, University of Paris Diderot, Paris, France Exposure to Aromatic Amines and Their Metabolites: Impact on Their Metabolism
11:30 - 12:00	Winnie Ng, University of Toronto, Toronto, Canada Mechanisms of Aromatic Amine-Induced Hematotoxicity
12:00 - 12:30	Samantha Carlisle, University of Louisville, Louisville, KY, USA The Effect of a Human Arylamine N-Acetyltransferase 1 Specific Inhibitor and Curcumin or Resveratrol on the Proliferation of Breast Cancer Cell Lines
12:30	Close of Workshop Denis Grant, University of Toronto