Mohammad Ali Naqvi†††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††††† 994163418

 

 

 

 

UNI393Y1Y

2008

 

 

Supervisor: Prof. Rudolf Winklbauer.

 

 

The Role of XRhoA in vegetal rotation in Xenopus laevis, II

 

 

Vegetal Rotation in Xenopus laevis is an important force generating process during Gastrulation that plays an important part in tissue separation at the Brachetís cleft, as well as in the internalization of the mesoderm. XRhoA plays a major role in vegetal rotation since a dominant-negative form thereof significantly blocks vegetal rotation. Inhibiting Rho-Activated Kinase (ROK) also causes the same effect. The blastopore never closes in these embryos. Here we show that the DN-XRhoA-induced phenotype can be partially rescued by countering the effects of the Dominant Negative with three times more amount of the Wildtype RNA. Moreover we also found that inhibiting myosin just before vegetal rotation has a weak effect on vegetal cell upwelling. This suggests the involvement of additional players downstream of ROK in the pathway that causes vegetal cell upwelling during gastrulation.

 

 

INTRODUCTION

 

MATERIALS & METHODS

 

RESULTS

 

FIGURES

 

DISCUSSION

 

REFERENCES

 

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