The application of the Quantum Dots

1. Application in Biomedical and Biological

For cell labelling, tracking cell migration, flow cytometry, fluorescence in situ hybridization, whole-animal contrast agents in vivo, pathogen detection, genomic and proteomic detection, fluorescence resonance energy transfer (FRET) sensors, and high-throughput screening of bio-molecules.(Ref. from: Klostranec, Jesse M. ; Chan, Warren C. Quantum Dots in Biological and Biomedical Research: Recent Progress and Present Challenges. Advanced Materials , Vol.18 (15), p1953-1964,2006)

a) QDs Barcode application for blood-borne pathogen detection (Ref. from J. M. Klostranec, etc. Convergence of Quantum Dot Barcodes with Microfluidics and Signal Processing for Multiplexed High-Throughput Infectious Disease Diagnostics. Nano Letters, Vol.7(9) , p2812-2818 , 2007)

b) Cell Labeling and Imaging (Ref. from Betty Y. S. Kim, Wen Jiang, Biodegradable Quantum Dot Nanocomposites Enable Live Cell Labeling and Imaging of Cytoplasmic Targets.Nano Letters, Vol.8, No.11, P3887-3892, 2008)

c) QDs beads application in Flow cytometry

1.1. The QDs surface modification technology

In order to apply in Biology, QDs must be biologically compatible. i.e. It is water soluble, no cytoxicity and stable in biological environment. Because the nanocrastal is toxicity for cells and tissues, so a very important step should be run. That is the nanocrastals must be coated with the polymers and proteins. The functionalized layer with COOH,NH2,OH and SH group can conjugated with DNA, antigen, antibody or some specific proteins, such as Streptavidin and biotin, to realize the applications we need, including DNA sequence and ELISA.

Lysine-MUA-QDs

MAA-QDs

BSA-QDs

PEG-QDs

PLGA-QDs beads

Reference:

• Klostranec, Jesse M. ; Chan, Warren C. Quantum Dots in Biological and Biomedical Research: Recent Progress and Present Challenges. Advanced Materials , Vol.18 (15), p1953-1964,2006
• Anderson, R.E. and Chan, W.C.W, Systematic Investigation of Preparing Biocompatible, Single, and Small ZnS-Capped CdSe Quantum Dots with Amphiphilic Polymers. ACS Nano, Vol.2(7) , p1341, 2008

1.2. Barcode beads making technology

a) QDs barcodes Beads

b) Fe3O4 magnetic particles and QDS barcodes beads

2. Application in Electronics and Energy industry

Organic LED for light, TV and cell phone screen. Solar electric panel (Photovoltaic Panel )

Flexible OLED Screen